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A quick summary: Trichromes, biosynthesis of THC, & Indica vs Sativa
Old 10-03-2008, 05:21   #1
psychedelicious
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Weed A quick summary: Trichromes, biosynthesis of THC, & Indica vs Sativa

There seems to be an ungodly amount of confusion over THC, its physical location in the cannabis plant, and the purpose of it / trichromes. I hope this post will help out those not in the know to figure out what's going on with THC and trichs.

Trichromes and Cannabinoid Production

Trichromes are glandular hairs which are found on many different plants. We lucked the fuck out with bud - its trichromes are quite a bit of fun!

Trichromes serve two main purposes:
1) They are sticky and aid in catching male pollen. More trichromes = better chance of pollination.
2) THC absorbs UV-B light very well, acting as a veggie sunscreen. THC is produced in the trichromes.

(It is thought that the trichromes and cystolith hairs, which we will get to in a minute, on herb might also play a role in deterring animals from eating the plants by making them less palatable, however, anecdotal evidence suggests that many animals find bud to be DELICIOUS. Humans are included in "many animals". )



This macro shot shows the different types of trichromes as well as tiny hairs (called cystolithic hairs - "hairs" whose cells contain mostly calcium carbonate).

The red arrow points to a cystolithic hair. Note: cystolithic hairs are not the little red hairs we all know and love. Those red ones are way bigger than cystolithic hairs, which, in cannabis, at least, are unicellular.

The yellow arrows point to bulbous glands, which are very, very small (2 to 8 cells big), and contain minimal amounts of cannabinoids. They are one of three types of glands.

The blue arrows point to capitate-sessile glands, which eventually mature into the third and last type of gland: capitate-stalked, which are indicated by the green arrows.



In this photo, you can see the tops of capitate-stalked glands, and perhaps some capitate-sessile glands. The bulbous glands are too small and too blurred-out to see in this photo.

Capitate-stalked glands are what most people refer to as trichromes. At the bottom of these glands are various phenols and turpenes, which are precursors to cannabinoids. The "head" of the capitate-stalked gland contains the cannabinoids. Gland age and THC content are directly related (older = more THC). As the phenols and turpenes are moved up the stalk and into the "head", they are progressively biosynthesized into cannabinoids: first to CBG, then CBG > CBC AND CBG > CBD, and finally, CBD > THC. CBN, the compound which THC primarily degrades to, forms only after the plant has died. The image below shows the biosynthesis progression.


1 = CBG (cannabigerol)
2 = CBD (cannabidiol)
3 = CBC (cannabichromene)
4 = THC (delta-9-tetrahydrocannabinol)

These glands grow up to half a millimeter tall. They are the ones you want, and they grow all over the aerial parts of the plant (anything above ground).

Here's a good read from the UN's Office on Drugs and Crime: LINK
And the wikipedia article on trichromes: LINK

Indica vs Sativa and Different Highs

So why do different pots have different effects? It has to do with the ratio of CBD and THC at the time of harvest. Most of the effects of herb are attributed to THC. When CBD (which has little to no effect on its own) is taken in conjunction with THC, it adds sedating and euphoric qualities to the high.

The time it takes a sativa plant to reach maturity is quite a bit longer than the time indica requires (once flowering has begun, 10-16 weeks for sativa compared to 6-8 weeks for indica). This makes all the difference in the high! Scroll back up to the graphic depicting the progression of cannabiniods. The longer a plant is alive, the longer its resin glands have time to take the steps to get from CBG > CBD > THC. Thus, as time goes on, the ratio of THC to CBD increases (meaning more THC and less CBD). This also means that plants that reach maturity faster have a lower ratio of THC to CBD (meaning less THC and more CBD).

Putting it all together...
Since sativas take quite a bit longer to reach maturity, they have more time to build up THC and have less CBD. This gives sativas a cerebral, psychedelic, and stimulating high - trippy buds.

Indicas, on the other hand, reach maturity much sooner than sativas, and so have less time to build up THC and have more CBD, giving indicas an extra bit of euphoria and sedation - couchlock buds.

For example, a sativa plant might have a THC:CBD ratio of 3:1, while an indica plant might have a THC:CBD ratio of 1:2.

Ah, and then there is ruderalis, which, it appears, has high levels of CBD, but no THC (or at least very, very little - not enough to get you high at all). Somebody with access to ruderalis ought to do a butane extraction and mix in some of the resultant oil with a bowl of straight sativa to see if the high is sedating instead of stimulating.

Another cannabinoid which is found primarily in Indian, Nepalese, S. African, and Afghani indicas is tetrahydrocannabivarin (THCV). THCV actually antagonizes (reduces, in this particular case) the effects of THC. This could possibly increase the sedating and euphoric effects of indica pots from these parts of the world, which are the varieties from which hashish is traditionally made.


Hope that clears a few things up!


Last edited by psychedelicious; 03-06-2008 at 05:57.. Reason: added some stuff and fixed a mistake
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Old 10-03-2008, 05:27   #2
TheRiseIsTheFall
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good post.

Fyi all you growers for that wonderful medicinal high on most indicas it's wise to wait until the trichomes turn a amber colour(couchlock anyone?)


Mmmmmmmm, Looking at this pic makes me hungry.
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Old 12-03-2008, 01:47   #3
keithwired
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yes amber colored trichomes means a higher CBN concentration whereas whiter or clearer trichomes means higher THC concentration = sativa cerebral head high
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Old 12-03-2008, 06:36   #4
Drack
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Very interesting... thanks.
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Old 12-03-2008, 06:37   #5
drscience
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This should be a sticky, it clears up alot
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Old 12-03-2008, 06:42   #6
theWorldWithin
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Quote:
Originally Posted by psychedelicious
As the phenols and turpenes are moved up the stalk and into the "head", they are progressively biosynthesized into cannabinoids: first to CBC, then to CBG, then to CBD, and finally to THC. CBN forms only after the plant has died.
THen why do growers harvest plants early for a more cerebral sativa type high? I have heard before that this synthesis occurs in the above order so maybe its a fairly quick process. Does THC degrade back to CBD in vivo while the plant is still alive? If not then why do plants in the earlier stages of maturity have a higher THC to CBD ration (while trichomes are still clear)?

By the way, nice to see some intelligent discussion in this forum for a change!
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Old 12-03-2008, 16:31   #7
psychedelicious
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keithwired, I believe you mean CBD, not CBN?

theWorldWithin, that's a good question. AFAIK, THC does not degrade back into CBD, though, once the plant has died, THC does degrade into CBN. I'm not sure about plants in the earlier stages having a greater THC:CBD ratios...

Perhaps at the start of growth, the plant rushes to make THC to block the new leaves and growth from the sun, until the plant gets a bit larger and can deal with the heat better. Then, during the middle stages, it slows this process down, and the ratio decreases, until the end when it speeds up to protect the buds from sunlight? Let's figure this out.

btw, I added an indica vs sativa section and an image showing the cannabinoid progression.
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Old 12-03-2008, 16:39   #8
MyDoorsAreOpen
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Quote:
Originally Posted by TheRiseIsTheFall
Mmmmmmmm, Looking at this pic makes me hungry.
Yeah that's about my first and only thought. I read this post like I read the articles in Playboy.

All curious people are philosophers. All seekers of meaning are spiritual. P&S
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Old 12-03-2008, 16:53   #9
psychedelicious
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Quote:
Originally Posted by MyDoorsAreOpen
Yeah that's about my first and only thought. I read this post like I read the articles in Playboy.
Pshhhhh :P

There's more to herb than pretty flowers!
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Old 14-03-2008, 23:35   #10
double ewe
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Thanks for posting! I find myself bringing up this thread on peoples' computers about every time I smoke these days.
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Old 15-03-2008, 00:25   #11
Slay
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awesome thread psychedelicious thank you i would love to read more about cannabinoids. also it would be nice to read some cannabinoid biotrasformation
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Old 15-03-2008, 07:17   #12
psychedelicious
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I'm glad you two enjoyed it! Makes my high feel way more warm and fuzzy than usual

I'll work on that, Slay.
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Old 30-05-2008, 10:10   #13
neverstop
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Quote:
contain inorganic compounds, mostly calcium carbonate).]
que?
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Old 30-05-2008, 14:31   #14
Czar420
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Quote:
Originally Posted by psychedelicious
Perhaps at the start of growth, the plant rushes to make THC to block the new leaves and growth from the sun, until the plant gets a bit larger and can deal with the heat better.
So does that mean one could germinate and sprout a few hundred baby plants and whack them down while they are young for the "nutrients". Much like how people eat baby spinach?
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Old 01-06-2008, 09:09   #15
time traveler
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Top stuff psychedelicious !

Funny how some people i know still think they are smoking the leaf.

At the other end now i can trump wikipedia drug nerds with a single treatise on THC biosynthesis.

Cheers
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Old 01-06-2008, 13:47   #16
centro
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great post dude
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Old 01-06-2008, 15:47   #17
Riddle
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Pornographic and informative, good post
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Old 03-06-2008, 06:16   #18
psychedelicious
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Quote:
Originally Posted by neverstop
que?
duh. thanks
Quote:
Originally Posted by Czar420
So does that mean one could germinate and sprout a few hundred baby plants and whack them down while they are young for the "nutrients". Much like how people eat baby spinach?
I would be willing to bet that baby pots are more nutritious than mature pots.
Quote:
Originally Posted by time traveler
Top stuff psychedelicious !

Funny how some people i know still think they are smoking the leaf.

At the other end now i can trump wikipedia drug nerds with a single treatise on THC biosynthesis.

Cheers
Quote:
Originally Posted by centro
great post dude

Quote:
Originally Posted by Riddle
Pornographic and informative, good post
muchas gracias!
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Old 02-11-2008, 19:13   #19
BuddyBoy
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hey i hopped over from Planet ganja, GREAT article! much appreciated. BUT I disagree on a fact posted here. You claim that the blue arrowed glands "grow" into the green arrowed glands? Like a mushroom? And the red arrowed gland is another type of gland?

In my opinion(15yr Harvester) thats a photo of an "early" ripe section of a calyx. All my experience under the scope shows glands like the red arrowed glands early in maturity and their tips form the heads as they swell and mature and eventually form the green arrowed structures. The stalks of these glands grow in gerth as well during the ripening process.
This observation is reinforced by the fact that there arent ever any "short" glands "growing into stalks.
I have seen small balls on the ends of glands though.

A stop frame reference of one particular section of a ripening bud would be of great help here but a complete pain to film.
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Old 02-11-2008, 19:23   #20
whoremoaning
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Good post, its a shame so many smokers don't know the difference between a sativa and an indica.
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Old 02-11-2008, 20:40   #21
psychedelicious
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thx whoremoaning!

BuddyBoy, the blue arrowed glands are the capitate-sessile glands, which are like the ball/head of a mushroom, if you cut the stalk off. They mature into capitate-stalked glands as a "trunk" grows from underneath, giving them that mushroomy look. The red arrowed glands are not glands, but cystolithic hairs. They are made up of calcium carbonate (CaCO3). They are kinda like mineral deposits, not glands, and definitely not cannabinoid-containing glands. Maybe they capitate-stalked glands grow up real quick? lol. You can see some shorter mushroomy stalked glands in the pic if ya check close up. I'll do more research and post back. thx for coming over to BL! this is the an AMAZING community, buddyboy, and it sounds like you are just the type of stoner we love over here
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actually in vivo it's CBG to THC... in vitro CBD to THC....
Old 28-05-2009, 00:16   #22
greenstoner
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actually in vivo it's CBG to THC... in vitro CBD to THC....

Hi there seems to be some confusion here... in vivo CBG - cannabigerolic acid, is synthesized into THC merci a THC synthase... you can read the rest of this at greenstonepatent.com (where we patented the in vivo mechanisms and show the route for setting them into bacteria....), best regards,
the decent folks at
GreenstonePatent.com and Enzymatic-THC.com
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Old 22-02-2010, 20:36   #23
Rude Rabbit
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What is the process that activates THC? Can the body be made to produce its own THC in order to render someone permanently high? Is that possible?
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Old 22-02-2010, 21:10   #24
greenstoner
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THC-A (tetrahydrocannabinolic acid) is produced by cannabis, and is activated by heat - i believe it's anything in the 105-110 degrees farenheit - and yeah, you should be able to catch a decent buzz by replacing the bacteria in your stomach with genetically modified e coli...access to a genetic engineering lab, skills, and ~$1,000 or so should do it
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Old 22-02-2010, 21:46   #25
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^ no thanks



great post btw
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